Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Microcomputed tomography (microCT) reconstruction of cortical and trabecular bone. MicroCT enables high-resolution three-dimensional reconstruction of microstructural characteristics from trabecular architecture to cortical porosity. (A) Trabecular bone from femoral neck of 51-year-old male (left) and 84-year-old female (right). (B) Diaphyseal femoral cortical bone of 18-year-old male (left) and 73-year-old female (right). Age, gender, disease, and other factors influence the microstructural properties of both cortical and trabecular bone, and these can be evaluated quantitatively by microCT.

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: Tomography

Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Contrast-enhanced imaging of mechanical load-induced bone microdamage in rat femora. Samples were loaded in three-point bending to 5% or 10% reduction in secant modulus and stained by barium sulfate (BaSO 4 ) precipitation. Load-induced microcracks provide nucleation sites for barium and sulfate ions to accumulate. BaSO 4 signal featured a linear attenuation coefficient approximately three times that of rat cortical bone, enabling co-registration of microdamage by microcomputed tomography. Reproduced with permission from Elsevier .

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: Imaging, Staining, Tomography

Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Correlation of local tissue strains with regions of bone formation and resorption by longitudinal in vivo microcomputed tomography (microCT) and finite element (FE) analysis. The sixth caudal vertebra of mice were loaded by pinned compression of the fifth and seventh vertebrae, resulting in a cyclic load of 9 N. Serial, co-registered microCT scans were analyzed to determine locations of bone formation and resorption (A) and correlated with locations of high/low strain energy density (SED) (B) , calculated by FE analysis. Bone formation and resorption were significantly more likely in regions of high and low SED, respectively. Reproduced with permission from PLOS .

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: In Vivo, Tomography

Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Microcomputed tomography (microCT) analysis of tissue engineering scaffolds and bone regeneration in vivo . (A) MicroCT reconstruction of mesenchymal stem cell-mediated bone regeneration in a bilateral cranial defect model treated with a high-density human mesenchymal stem cell (hMSC) construct incorporating growth factor-loaded microparticles (left) or empty control (right), and sagittal-cut views of three-dimensional thickness mapping overlay of defect regions (Phuong Dang and Eben Aslberg, in preparation). (B) Poly(L/DL)-lactide tri-calcium phosphate (PLDL-TCP) scaffold created according to the protocol of featuring oriented microporosity and central core showing isometric view and transverse cross-section. (C) In vivo bone formation in a rat femoral bone defect model implanted with cored scaffolds from (A) loaded with rhBMP-2 in alginate hydrogel (McDermott and collegues, in preparation) or (D) hydrogel-mediated delivery of rhBMP-2 within a polycaprolactone nanofiber mesh without a structural scaffold in the defect. Reproduced with permission from . (E) In vivo microCT-based longitudinal quantification of bone formation over time (dotted to solid lines) for various doses of BMP-2 in the hybrid nanofiber mesh/alginate delivery system. Reproduced with permission from .

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: Tomography, In Vivo, Construct

Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Microcomputed tomography angiography analysis of hindlimb ischemia recovery. (A) Three-dimensional reconstructions of distal hindlimb vasculature in wild-type (WT) (left) and MKP-1 knockout (KO) (right) mice at day 7 post-surgery. Reproduced with permission from the American Heart Association . (B) Quantification of vascular volume ratio (ischemic/contralateral control), illustrating the biphasic nature of angiogenic and arteriogenic vascular recovery. Initial conditions (T 0 ) taken from comparable WT C57Bl6 mice in , with continuity indicated by gray lines. Horizontal line illustrates ischemic/control ratio of 1.

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: Tomography, Knock-Out

Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Microcomputed tomography (microCT) angiography of vascular response to bone injury and regeneration. (A) MicroCT image of age-matched unoperated rat femur with surrounding vasculature: the large vessels on the right-hand side are the femoral artery and vein. (B) Vascular structures and bone ends 3 weeks after creation of an 8-mm bone defect. There is a robust neovascular response to bone injury, characterized by increased branching, new vessel formation, and decreased degree of vascular anisotropy. (C) MicroCT image of a nanofiber mesh wrapped around a bone defect. Bone formation has occurred on the surface of the mesh, highlighting the mesh perforations. (D) MicroCT angiography was used to visualize radially directed vascular ingrowth from the surrounding soft tissue envelope through the nanofiber mesh perforations, illustrating the contribution of soft tissues to regenerate bone vascularization and the importance of biomaterial porosity. Owing to the overlapping attenuation of bone and the lead chromate contrast agent, separate segmentation within the same sample is not possible without extensive post-processing and image registration. Images reproduced with permission from the National Academy of Sciences (frames A and B) and from Elsevier (frames C and D).

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: Tomography

Journal: Stem Cell Research & Therapy

Article Title: Microcomputed tomography: approaches and applications in bioengineering

doi: 10.1186/scrt534

Figure Lengend Snippet: Equilibrium partitioning by ionic contrast-agent microcomputed tomography (EPIC-microCT). Experimental osteoarthritis was induced by intra-synovial injection of monosodium iodoacetate (MIA) and evaluated at week 3 by EPIC-microCT. Excised rat femurs were equilibrated with an ionic contrast agent (hexabrix) and scanned to assess cartilage and subchondral bone. The arthritic group exhibited substantial sulfated glycosaminoglycan depletion, cartilage degradation, and subchondral bone resorption, illustrating the capacity of contrast-enhanced microCT to quantitatively assess cartilage and bone in preclinical models of osteoarthritis. Arrows indicate location of complete cartilage degradation in MIA group and corresponding location in the control. L, lateral; M, medial. Figure reproduced with permission from John Wiley & Sons, Inc. .

Article Snippet: Recently, Tapfer and colleagues, in collaboration with Bruker® MicroCT (Bruker Corporation, Billerica, MA, USA), described a rotating gantry phase-contrast microCT system based on a polychromatic x-ray source [ ] that they have applied to ex vivo scans of a murine pancreatic tumor model with soft tissue contrast similar to MRI [ ].

Techniques: Tomography, Injection

Journal: bioRxiv

Article Title: Tailoring a glycerol-free cryopreservation protocol with anti-freeze (glycol)proteins for commercial and native breeds of chicken

doi: 10.1101/2025.07.18.664799

Figure Lengend Snippet: Identification of very early (oviductal) embryo death. Germinal discs were gently removed from the vitelline membrane, immersed in 0.96 % NaCl and stained on microscope slides with propidium iodide. The presence of cell nuclei is an indicator of fertility. Unfertile germinal disc (left) and fertile germinal disc (right). (Photographs taken by Dr. Krisztina Liptói, NBGK)

Article Snippet: The slides where mounted and analysed under a phase contrast microscope (Zeiss Axioskop 2 plus, camera Micro Publisher 6, Canada) at 1000× magnification.

Techniques: Membrane, Staining, Microscopy